CBMAIgrown in artificial seawater were able to catalyze the hydrolysis of benzyl glycidyl ether Martins et al. Aim To isolate and characterize fungal strains capable of degrading profenfos.
Growth of marine fungi A. The result is the formation of a strong hydrogen bond between a protonated histidine residue of the catalytic site and the negatively charged oxygen atom of the inhibitor.
Synthetic pesticides are recognized as a cost-effective method of controlling pests, improving productivity and food quality. Detoxification of pesticides in situ has been achieved by treatment of the contaminated soil with certain microorganisms or plants, a technology known as bioremediation or more specifically, phytoremediation in the case of plants Sutherland et al.
Profenofos, O- 4-bromochlorophenyl O-ethyl S-propyl phosphorothioate, is a broad spectrum, non-systemic foliar insecticide and acaricide. Various benefits are derived from the use of pesticides, however they also cause a wide array of toxicological and environmental problems and often persist in the environment for long periods of time.
Isolation procedure The isolation method of Lansing M. Profenofos residues, extracted in hexane from salt media or from the soil samples, were analyzed in Agilent Gas chromatograph Model Series USA equipped with a Ni 63 electron capture detector with HP-5MS column length 30 m, diameter 0.
Two small slices of solid medium 1. The resulting colonies were repeatedly subcultured in the same medium FTW with mgL-1 of profenofos to confirm their profenofos utilizing ability. The residual no degraded profenofos and the 4-bromochlorophenol released were analyzed by a gas cromatography coupled to a mass spectrometer in single-ion monitoring mode GC-MS-SIM.
Published material is freely available to all interested online readers. In the literature there are some cases of bio degradation of organophosphate pesticides that occur by subsequent biotransformations, yielding novel polar metabolites, such as glycosylated and sulfated derivatives.
Duplicate samples of 1. Profenofos, Growth kinetics, Biodegradation, fungal isolates. Triplicate sets of each composition without inoculation were kept as controls.
Aliquots of this suspension were streaked on FTW profenofos agar plates. However, the mechanisms of fungal degradation of these compounds are less established than those used by bacteria, since there are few studies on fungal degradation of OPs.
The extract was filtered and the organic solvents were evaporated to near dryness on a rotary thin film evaporator. Determination of profenofos concentration in commercial sample The sample was prepared with The results are summarized in Table The agar was allowed solidify and a wedge shaped layer was obtained.
All the other reagents used in this study were of high purity and analytical grade.
The 4-bromochlorophenol was purchased from Sigma-Aldrich, Brazil. Specific bioremediation of OPs requires highly specialized enzymes, so genetic engineering has been used to improve the properties of enzymes from various sources to enhance catalytic rates, stability and substrate range Sutherland et al.
The growth performance was recorded in terms of the length of the fungal growth in centimeters across the profenofos gradient plates. Being an international journal focused on Engineering, Management, Science and Mathematics, we broadly cover research work on next generation cutting edge technologies and effective marketing strategies.
M, Caroline Jessica Solomon, V. Identification of the isolates The morphological, physiological and biochemical tests of isolates W and Y, were performed by using standard methods. This model provides immediate, worldwide, barrier-free access to the full text of research articles without requiring a subscription to the articles published in this journal.
The biodegradation of profenofos in the presence of these selected fungi was evaluated, assessing the degradation of the pesticide, as well as the formation of the metabolite, 4-bromochlorophenol.
It is effective against a wide range of chewing and sucking insects and mites on various crops Reddy and Rao, To obtain pure cultures of single strains, 5 mL aliquots of enrichment cultures were centrifuged at rpm for 5 min TGLA centrifuge, Changsha Pingfan instrument Ltd and the cell pellets were resuspended in 2 mL sterile media.
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The marine fungi were isolated and purified in the microbiology laboratory of the Department of Ecology and Evolutionary Biology supervised by Professor Mirna H. The MIC of profenofos determined for the fungal isolates was in accordance with the gradient plate results.
After extractions, the organic phase was dried over anhydrous Na2SO4, followed by solvent filtration and evaporation, resulting in a final volume of The samples aliquots of liquid culture as well as soil were subjected to organic solvent extraction three times with acetone: The isolates had the greatest length of growth in the range of 1 to 1.
Strains named as W and Y degraded profenofos were subejected for further studies and are presented in this study. The concentration of internal standard, chlorpyrifos, was maintained at Aliquots of this suspension were streaked on FTW profenofos agar plates.
Marine enzymes have a great potential for use in biocatalytic reactions, as in biodegradation of pesticides, due to the peculiar characteristics of the marine environment. Among fungi involved in the degradation of cellulose, the most active belong to the fungal genera of Aspergillus, Penicillium, Trichoderma, Alternaria and Chaetomium (Krakov a et al., b), but.
Asian Journal of Science and Technology (AJST) is a monthly, open access, peer-reviewed and fully refereed international journal.
Being an international journal focused on Engineering, Management, Science and Mathematics, we broadly cover research work on next generation cutting edge technologies and effective marketing strategies.
An enrichment culture technique was used to isolate bacterial strains responsible for the biodegradation of profenofos in a soil from Hubei province of central China.
Biodegradation Process mL flasks were used to carry out Biodegradation,for this purpose two strain of specified isolates fungal (Aspergillus sp.
and Alternaria sp), were grown on the gasoil samples (diesel) from pajji refinery. Asian Journal of Science and Technology (AJST) is a monthly, open access, peer-reviewed and fully refereed international journal.
Being an international journal focused on Engineering, Management, Science and Mathematics, we broadly cover research work on next generation cutting edge technologies and effective marketing strategies. Bioremediation of profenofos-contaminated soil was examined using soil treated with ug g-1; profenofos resulted in a higher degradation rate than control soils without inoculation.
In a mineral salt medium (FTW) reduction in profenofos concentration was 90% within 96 hours of incubation.Biodegradation of profenofos using fungal isolates